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KMID : 0613820230330050414
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Journal of Life Science
2023 Volume.33 No. 5 p.414 ~ p.421
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Effect of Cnidium japonicum Miq. Crude Extracts on UVB-induced Photoaging Damage in Human Keratinocytes
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Lee Eun-Seong
Oh Jung-Hwan
Kong Chang-Sukg
Seo Young-Wan
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Abstract
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Cnidium japonicum (C. japonicum) is a type of halophyte that inhabits soil of a high salinity, and according to previous studies, it is known to have antitumor effects. However, the skin’s protective effect, particularly against UVB irradiation, has not been revealed. In this study, C. japonicum crude extract was studied to determine its effect on damage to human keratinocytes (HaCaT) induced by UVB irradiation, and ROS assays were performed, the results of which showed that C. japonicum crude extract affects UVB-induced photoaging damage in human keratinocytes. To examine inhibitory effects against the expressions of MMPs, RT-PCR and Western blot assay were performed by treating the crude extract at concentrations of 10, 50, and 100 μg/ml by irradiating UVB at 15 mJ/cm2. As a result, it was confirmed that the mRNA and protein expression levels of MMP-1, MMP-3, and MMP-9 decreased in the group treated with C. japonicum crude extract, which also effectively regulated the antioxidant defense mechanism pathway by activating JNK, ERK, and p38. In conclusion, the current study suggested the possibility that C. japonicum could be used as a raw material for anti-photoaging cosmeceuticals in the future.
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KEYWORD
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Cnidium japonicum, human keratinocytes, MMPs, photoaging damage, UVB
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